Benchmark run 2026-03-13

LFQBench · Orbitrap Astral AWS c7i.8xlarge 17.41 h

185,728 Precursors

14,267 Protein groups

6 Raw files analysed

Quantification accuracy was not recorded for this run — the LFQBench analysis was added to the pipeline later. See the run list for runs that include it.

Per-file Results

Each of the 6 raw files, analysed independently. Mass and retention-time errors indicate calibration quality.

File	Precursors	Proteins	MS1 error	MS2 error	RT error	FWHM (RT)
`A_R1`	147,306	13,420	1.0652	5.904	410.8784	2.9147
`A_R2`	174,480	13,861	0.7093	7.5235	116.7446	2.9056
`A_R3`	158,757	13,680	0.8632	5.9808	165.8319	2.7775
`B_R1`	158,546	13,835	0.7603	6.4261	355.5752	2.9228
`B_R2`	171,282	13,936	0.7698	6.0122	85.6897	2.7941
`B_R3`	171,543	14,054	1.2086	11.7601	205.448	3.0416

How this was measured

LFQBench is a community-standard benchmark for label-free quantification. Because the mixing ratios are fixed when the samples are prepared, the correct answer is known independently of any software — which makes it possible to measure accuracy rather than merely report output volume.

Dataset

Orbitrap Astral — 6 raw files: conditions A and B, 3 replicates each.

Ground truth

Expected log₂ ratios — Human 0.0, E. coli -2.0, Yeast 1.0.

Environment

AWS c7i.8xlarge, single run. Random seed fixed so the analysis is reproducible.

Scope

Measured on the main branch of the SynapSpec engine. Runtime reflects one run on shared infrastructure and will vary with hardware.

Benchmark run